Peripheral blood clues to nonneoplastic conditions online ce course. Changes in white blood cells in sheep blood during selenium. A new method of assessing sheep red blood cell types from their morphology ivona zura zaja 1, silvijo vince 2, nina poljicak milas 3, ingo ralph albin lobpreis 4, branimira spoljaric 2, ana shek vugrovecki 1, suzana milinkovic. White blood cells may exhibit several morphological changes, and the neutrophils are the cell type primarily affected. The complete blood cell count cbc cbc part 1 the hemogram. Before we start with the abnormal morphologies, lets talk about normal morphology of red blood cells. Normal white blood cell morphology segmented neutrophil segmented neutrophils are the most common white blood cells in peripheral blood of all the common domestic. When a peripheral blood smear is made for the purpose of evaluating rbc morphology, accurate recognition. The marked poikilocytosis shown in this blood sample is commonly a. Summary of abnormal red blood cell morphologies and disease states 5 years ago by medical labs 0 before we start with the abnormal morphologies, lets talk about normal morphology of red blood cells. In wellspread, dried and stained films the great majority of cells have round, smooth contours and diameters within the comparatively narrow range of 6. Blood cells can be identified in blood films stained with a mixture of basic and acidic dyes. It is limited to the material covered in the lectures and laboratory sessions. In liquid culture media like trypticase soy broth or nutrient broth, the growth of the bacterium occurs as turbidity in the broth medium but the chances of contamination are high especially when the isolation is carried out from feces specimen, commonly the selenite f broth and tetrathionate broth is used for the isolation of salmonella typhi.
Morphometric parameters of rbc size and shape were determined from stained blood smears using sform, a computerassisted program for automated cell morphometric measurements. Red blood cell storage and cell morphology 93 storage 42 days, haemolysis was lower than the 0 8% threshold for all the tested subjects 0 34 0 1 fig. Myeloblasts ie, the earliest precursor originate in the marrow from a stem cell common to erythroid, megakaryo cytic, and granulocytic cells. It brilliantly illustrates an even broader spectrum of morphologic variation in red and white blood cells. Unfortunately, a few cell illustrations are not available at this time but will be added.
Dorothy sturms fantastically detailed watercolors depict human blood cells in every stage of development and pathologic change. Peripheral blood clues to nonneoplastic conditions. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Blood cells an atlas of morphology with clinical relevance pdf. Blood types or groups are determined by specific antigens found on the surface of erythrocytes. The page below is a sample from the labce course red cell disorders. In man and in all mammals, erythrocytes are devoid of a nucleus and have the shape of a biconcave lens. Pdf a new method of assessing sheep red blood cell types. Nevertheless, it is essential to fill the tube exactly to the mark, so smaller tubes may be unavoidable for small patients. Thermo scientific columbia blood agar with sheep blood medium. Most of these neutrophilic changes originate in the cytoplasm in response to various pathologic processes. Segmented neutrophils are typically 10 to 12 11min diameter and have single nuclei with several indentations resulting in the.
Data concerning the morphometric parameters of sheep red blood cells rbcs obtained using computerassisted image analysis have not yet been investigated, and there are no data on any analyses of ovine rbc subpopulations based on their morphometric parameters. The sheep haematological profile includes the determination of haematocrit hct or packed cell volume pcv, of haemoglobin, of red blood cell indices, of total cell counts, of differential cell counts and the evaluation of erythrocyte, of leucocyte and of platelet morphology by microscopic examination of stained blood films kramer, 2000. In this article we will discuss about escherichia coli e. In general, nuclear convolution in sezary cells is more delicate and finer than in the neoplastic atl cells. Red blood cells rbcs, also referred to as red cells, red blood corpuscles, haematids. Hematology cell morphology red blood cells normal erythrocytes normal goat erythrocytes normal goat erythrocytes. If you continue browsing the site, you agree to the use of cookies on this website. Tutorial blood cell morphology a clinical pathology 201. Haematological and serum biochemical indices of sheep. Study of 95,141 complete blood count determinations. The influence of age and sex on the heamatological values of goats and sheep studied in the arid zone of borno state of nigeria.
Abnormal morphology of blood cells april 2012 hematologyimmunology system faculty of medicine universitas padjadjaran. Complete blood count using hmx nhanes 20072008 the coulter hmx hematology analyzer is a quantitative, automated hematology analyzers and leukocyte differential cell counters for in vitro diagnostic use in clinical laboratories. Normal mature rbc are biconcave, round discs that are about 6 8 in diameter, which is only slightly smaller than the normal small mature. They tend to block blood flow in the blood vessels of the limbs and organs.
However, if pathological or irregular results are discovered, or abnormal morphology or blood cell function impairment is suspected, an additional microscopic blood smear evaluation is strongly recommended. The presentation of illustrative cells in this module is by no means a comprehensive study of blood cells. Since the focus of the manual is on morphology, success depends on the quality of the illustrations. Abnormal results may vary depending on the type of blood cell affected. A new method of assessing sheep red blood cell types from their morphology ivona zura zaja 1, silvijo vince 2, nina poljicak. Clearly we can all appreciate the normal shape of a red blood cell. Aug 20, 2008 in hematologyoncology fellowships, fellows are taught blood cell and bone marrow morphology by counting and identifying thousands of cells in normal and abnormal smears, either by apprenticeship with a master or by comparison of cells, cell by cell, with an atlas. Haematological and serum biochemical indices of sheep insemi. Ivona zura zaja 1, silvijo vince 2, nina poljicak milas 3. There are two different kinds of white blood cells and each looks different from one another under the microscope. In humans, there is the abo system of blood types, whereas animals have a variety of different blood types. Using interactive multimedia ebooks for learning blood. Morphology of blood cells blood body fluids biology. White blood cells wbc are a heterogeneous group of nucleated cells that can be found in circulation for at least a period of their life.
Age and sex remarkable influence p blood cell rbc counts of goats. A new method of assessing sheep red blood cell types from. Red blood cell count rbc was significantly p of the bacterium occurs as dense turbidity in the broth medium with a surface pellicle which is further analyzed for the morphology under the microscope, gram reaction, biochemical tests, and pseudomonas aeruginosa specific tests. The cells are red cells or erythrocytes, white cells or leucocytes and platelets. Guidelines for reporting red blood cell morphology.
Blood is located in almost every part of the body, because it. Thermo scientific columbia agar with sheep blood promotes haemolysis typical of staphylococcus aureus, and it gives typical growth for streptococcus pneumonia dent morphology. Blood cell morphology in health and disease clinical gate. Milas 3, ingo ralph albin lobpreis 4, branimira spoljaric.
Bartlett, g patterns of phosphate compounds in red blood cells of man and animals. Wbc morphology deteriorates most quickly, especially in equine blood, so if the sample cannot be analyzed immediately, a thin blood smear should be submitted to the laboratory with the blood sample. Introduction to red blood cell morphology reporting. Difficult to identify true microcytes and rbc are not usually reported as microcytes in animals with mcv below the lower reference limit for that species. There are three types of cells in the myelocytic or gran ulocytic series. Pdf data concerning the morphometric parameters of sheep red blood. Home hematology abnormal red blood cells morphology and possible causes abnormal red blood cells morphology and possible causes. In health, the red blood cells vary relatively little in size and shape. Using interactive multimedia ebooks for learning blood cell. Rbc morphology reporting per high power field 400x few.
Sep 11, 2016 sheep erythrocytes have a diameter of 45. Collect appropriate blood volume and number of sets. Blocked blood flow can cause pain and organ damage. We compare the obtained results between local brown of atlas cattle, ouleddjellal sheep, arbia local goat, the arabian thor. The aims of this study are to determine the values of rbc haematological and morphometric size and shape. Below is a summary of terms used to describe red blood cell morphologic features, the meaning of the term and associated interpretation the provided list of diseases is not exhaustive. Rarely is a textbook so succinct, so wellorganized, and so beautifully illustrated as this. Granulocytes have visible granules or grains inside the cells that have different cell functions. Pdf on sep 11, 2016, nezar adili and others published species determination using the red blood. Data concerning the morphometric parameters of sheep red blood cells rbcs obtained using computerassisted image.
The neoplastic cells in peripheral blood and bone marrow smears may mimic sezary cells and may share the same immunophenotypic features expressing cd4 and pantcell markers except cd7. Abnormal morphology of red blood cell when a blood smear is inspected for abnormal morphology, two criteria must be met. In hematologyoncology fellowships, fellows are taught blood cell and bone marrow morphology by counting and identifying thousands of cells in normal and abnormal smears, either by apprenticeship with a master or by comparison of. The hemogram louisiana state university medical center department of pathology new orleans, louisiana by carolyn sue walters, mhs, mtascp a clinical pathology 201 study module click here to continue school of medicine 010703 do not reproduce this exercise. They play a most important role in phagocytosis and immunity and therefore in defense against infection. Composition of the blood the circulating blood is composed of plasma and cells. Fiftyone interns from three taiwan medical schools training by a single teacher in the pediatric hematology department of kaohsiung chang gung memorial hospital, taiwan, participated in this study. Their normal concentration in blood varies between 4000 and 10,000 per microliter. Summary of abnormal red blood cell morphologies and disease. Blasts are immature progenitor cells with characteristic morphology and cell surface markers. Soch 1faculty of agriculture, university of south bohemia, ceske budejovice, czech republic 2institute of parasitology of the academy of sciences of the czech republic, ceske budejovice, czech republic. Polymorphism of the haemoglobins of sheep and goats was first described in 1955 41,63. Abnormal red blood cells morphology and possible causes.
Sentinel laboratory guidelines for suspected agents of. The erythrocytes are the most numerous blood cells i. Summary of abnormal red blood cell morphologies and disease states. A blood smear is a blood test used to look for abnormalities in blood cells. This prospective study compares the use of interactive multimedia ebooks ime with traditional powerpoint tpp for teaching cell morphology of blood and bone marrow. A new method of assessing sheep red blood cell types from their morphology. Nov 14, 2016 this prospective study compares the use of interactive multimedia ebooks ime with traditional powerpoint tpp for teaching cell morphology of blood and bone marrow.
Besides transporting vital components, the blood plays an important role in the immune functions of the body and is vitally important to coagulation ability of blood to clot properly. Article a new method of assessing sheep red blood cell. Blood cell morphology free download as powerpoint presentation. Nov 12, 2016 blood cells an atlas of morphology with clinical relevance pdf slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Abnormal morphology of white blood cell white blood cells may exhibit several morphological changes, and the neutrophils are the cell type primarily affected. Guidelines for reporting red blood cell morphology labce. Compare sheep red blood cells from leading suppliers on biocompare. Knowledge of blood types in the different species is important as transfusion of incompatible blood the donor animal has a different blood. A new method of assessing sheep red blood cell types. Species determination using the red blood cells morphometry. Species determination using the red blood cells morphometry in.
Our goal is to obtain sheep rbc morphometric parameters as well as rbc subpopulations based on their morphometric parameters. The aim of the present work was to identify seasonal oscillations in the composition and morphology of the blood in intact rats. Morphometric measures of blood were determined in 36 clinically. The key word for it is biconcave disc, meaning that it is a disc shaped or circular cell that is compressed at the centre in both directions. Haematological tests in sheep health management sciencedirect. Changes in white blood cells in sheep blood during selenium supplementation l. Summary of abnormal red blood cell morphologies and. The white blood cell and differential count clinical. Sentinel laboratory guidelines for suspected agents of bioterrorism yersinia pestis american society for microbiology. Data were analyzed for the effect of breed, sex and season packed cell volume pcv was significantly higher p of the north west. Grow fastidious organisms with rapid production of large colonies, clearly defined zones of haemolysis and good colonial differentiation for allaround performance. Fiftyone interns from three taiwan medical schools training by a single teacher in the pediatric hematology department of kaohsiung chang gung memorial hospital, taiwan, participated in. Clinical hematology clinical pathology and procedures. Blood cells, 2nd edition gives you more on every page.