Micro propagation is vegetative propagation of plant using plant tissue culture. Tissue culture of banana tissue culture this is typically facilitated via the use of a liquid, semisolid, or solid growth medium, such as broth or agar, in vitro under sterile growing conditions. Banana, a water loving plant, requires a large quantity of water for maximum productivity. Commercial tissue culture involves exposing plant tissue to a specific regimen of nutrients, hormones, and light under sterile conditions to produce many new plants over a very short period of time. Agar is generally used at a concentration of 612 gliter. Sristi agro biotech pvt ltd, a company incorporated for setting up of a tissue culture production unit production.
Tissue culture banana,tissue culture plant,tissue culture. How to plant in clay, poorly draining, and compact soils. Tissue culture propagation of banana plant lets duration. Introduction plant tissue culture is culturing of any part of the plant in a specially defined growth media under aseptic laboratory condition in petri dishes, test tubes or in any other suitable glass containers. Tissueculture techniques established for banana include shoot and meristem culture, callus culture, somatic embryogenesis, cell suspension, and protoplast. Do not destroy the infected banana plant first as the banana aphids will fly off to the healthy plants and spread the virus. Plant tissue culture media, salt, and vitamins banana. Biotech class secondary education major in biological science doing banana tissue culture at romblon state university. In studies conducted on banana, apical meristems were. Individual banana plantlets ready for planting into plant pots or polybags. Media preparation plant tissue culture protocol sigma.
Plant culture tested pct naocl 4% in himedia is too costly 500 ml costs rs. Plant tissue culture techniques of banana slideshare. To reduce clumping, add powder slowly to the culture medium with constant stirring. These small pieces may come from a single mother plant or they may be the result of genetic transformation of single plant cells which are then encouraged to grow and to ultimately develop into a whole plant. Any number of samples less than 144 and arranged in a combinatorial format can also be indexed by this technique the technique developed by us for mass indexing of banana samples for bbtv is. Banana produced from tissue culture banana plants gives a better price in the market due to their quality and uniformity. Cultivation of tissue culture grande naine g9 banana. Agara polysaccharide powder derived from algae used to gel a medium. Chacko division of plant physiology and biochemistry, indian institute, of horticultural research, bangalore 560 080 india contribution no. In recent years there has been a spurt of activity in developing tissue culture protocols for this crop cronauer and krikorian,1986. Low cost media options for the production of banana musa. Seminar on technology transfer on tissue culture banana cultivation and market advocacy through federation of banana growers of kalakad, tirunelveli date. Plant from tissue culture or vitroplants plants are transplanted into larger bags and moved into a weaning nursery to adapt to higher sunlight and lower humidity found under field conditions.
Ratoon crop the main benefit of ratooning is that the crop matures earlier in the season is a unique feature of tissue culture banana plants. Banana tissue culture information guide for beginners. Stephan plant biotechnology laboratory, pg and research department of botany, govt. How to establish a tissue culture banana hardening nursery. Therefore under indian condition banana production should be supported by an efficient irrigation system like drip irrigation. Micropropagation for production of quality banana planting material in asia pacific. You need to read the threads and a few scientific papers and kind of go from there. Contamination in tissue culture can originate from two sources, either through carry over of microorganisms present on the explant surface or in the tissue itself endophytic microorganisms. In kenya, about 2,000 healthy banana plantlets are produced from a single shoot of tissue culture practice compared to ten suckers from a single banana plant in kenya within six months. The writer is chief executive officer, nsigotech tissue culture laboratory. Commercial cultivation of banana using plant tissue culture technique. Culture media used at different stages of banana micropropagation 50 iii. Product number b4032 is a powder from a spraydried mixture of banana and dextrin.
Home plant tissue culture media, salt, and vitamins banana medium banana medium show 15 20 50 100 200 500 999 results per page. Preparing tissuecultured banana plantlets for field planting. A piece of plant tissue is cut from the plant, disinfested, and. Plant tissue culture relies on the fact that many plant cells have the ability to regenerate a whole plant totipotency. A plant biotechnology laboratory manual 6 anjana r. We produce about 2 millions of tissue culture plants in our laboratory we have two acres of green house covering area for primary hardening and secondary hardening. Tissue culture banana in kenya produces faster and fruits within 340 days compared to. The planting time may be adjusted so as to avoid high temperature and drought at the time of emergence of bunches i. Growth of living plant tissues in a suitable culture medium in vitro is known as plant tissue culture. Download the book into available format new update. I want to sterilize my banana shoot tips with naocl in the initial stage of plant tissue culture in largescale. Important banana commercial varieties grown in asiapacific 49 ii. Pdf production of bananas in uganda using tissue culture. If you choose to plant 640 plants per acre, the plant spacing is 2.
Thus for efficient propagation of banana the invitro study of optimum combination of cytokinins and auxins and their interaction in a tissue culture medium for a spe cific cultivar is necessary. Tissue culture laboratory, plant and microbial sciences department. Tissue culture is collection experimental methods of isolation and inoculation of organs, tissues and cell in an artificial medium under invitro aseptic condition. The fertilizer schedule for tissue culture banana variety grand naine both in. Kande baug rabi month of planting october november. We are a commercial plant tissue culture company with other biotech sectors. Asiapacific consortium on agricultural biotechnology apaari. How banana farmers in kenya can benefit from banana tissue.
Tissue culture is exposing plant tissue to a specific regimen of nutrients, hormones and light under sterile conditions to produce many new crops, each a. To ensure farmers easy access to tc plantlets in various parts of the country hardening nurseries are critical. Some basic elements of banana cultivation banana musa acuminata or musa balbisiana is a giant herbaceous plant and is complete in its morphological characteristics, i. Planting of tissue culture banana can be done throughout the year as per the market demand except when the temperature is too low or too high. Single cells, plant cells without cell walls protoplasts, pieces of leaves, or less commonly roots can often be used to generate a new plant on culture media given the required nutrients and plant hormones. There are two important seasons in maharashtra, india. A revised medium for rapid growth and bioassays with tobacco. Micropropagation is one of the finest ways of plant multiplication by in vitro technique of plant tissue culture.
There are three main steps to the tissue culture process. Tissue culture plantlets are healthy, pest and disease free, uniform and shorter harvesting period. Project report on plant tissue culture biology discussion. How i propagate tissue culture banana seedlings daily nation. Bbtv free quality banana tissue culture plants can be ensured by using this technique. There are a number of reasons why tissue culture is the best method, however the three most obvious ones are. Banana fruits in kenya are extensively cultivated as a staple food and also as an income generating activity by many local communities. The propagation of a plant by using a plant part or single cell or group cell in a test tube under very controlled and hygienic conditions is called tissue culture. Fruit plants banana plant tissue culture, exporter from pune. Tissue culture tc techniques have been used in kenya over the last ten years to produce large quantities of diseasefree banana planting material. Exporter of fruit plants banana plant tissue culture, sugarcane plant tissue culture, pomegranate plant tissue culture and strawberry plant tissue culture offered by kala biotech private limited, pune, maharashtra. The product is plant tissue culture tested but it is the sole responsibility of the user to ensure the suitability. Banana plants produced from tissue culture are free from diseases at the time of supply and they give high yields.
Pdf banana plantlet production through tissue culture. Banana trees for commercial purposes are generally propagated by tissue culture. Tissue culture involves the use of small pieces of plant tissue explants which are cultured in a nutrient medium under sterile conditions. Virus testing protocols for banana adopted at nrcb 51 iv. Several banana tree cultures in the incubating process. The next article will focus on soil fertility, weed and water management in a banana plantation. Herbaceous plant tissue culture banana tree mreinhartpsu. If you plant 450 plants per acre, the distance between holes is 3 metres by 3 metres.
Step by step how to tissue culturing bananas there is a book you can buy, its called plants in a test tube or something lik that, but again doesnt specifically cover bananas. Planting time tissue culture bananas can be planted throughout the year. Plant tissue culture terminology adventitiousdeveloping from unusual points of origin, such as shoot or root tissues, from callus or embryos, from sources other than zygotes. In both adaptation phases, abnormal plants, with disease problems or slow growth must be. The newer tissue material obtained through r dna technology or haploid culture or somatic hybridization can be the source of tissue material for micropropagation, as it is the easiest method for obtaining the multiple propagules. A novel molecular technique for mass indexing of tissue. The agriculture plant we offer are grown on fertilize soil, under natural conditions.
We offer a banana powder for use in orchid and other plant cell culture. Banana micropropagation medium has been developed for the in vitro propagation of musa species, family musaceae. Culture medium is a nutrient medium which contains all essential micro and macro nutrients, carbohydrates, vitamins and hormones. Status in india banana is a globally important fruit crop with 97. Being a monocotyledonous crop it was thought to be intractable to in vitro techniques. Ideas behind and the advantages of tissue culture tissue culture of a plant relies on the fact that many plant cells have the ability to regenerate a whole plant which is termed totipotency. Uhctahr preparing tissuecultured banana plantlets for field planting bio8 mar. Suckers on the contrary are not uniform and have a rather longerharvesting period thus management of the plantation can be somewhat wearisome. Scientia horticulturae, 18 198283 247252 247 elsevier scientific publishing company, amsterdam printed in the netherlands tissueculture propagation of banana r. Mohan ram and steward in 1964 demonstrated the possibility of raising callus cultures from the fruit tissue of various genomes. Banana plantlet production through tissue culture article pdf available in pakistan journal of botany 363 september 2004 with 25,539 reads how we measure reads. Asiapacific consortium on agricultural biotechnology.